Ac-DEVD-AFC | CAS 201608-14-2 | Chemical-Suppliers

Element: devd

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C-S » Chemicals » devd-afc-PX94466

Identification

Ac-DEVD-AFC

CAS:

201608-14-2

EINECS:

Molecular Formula:

C₃₀H₃₄ F₃ N₅ O₁₃

MDL:

MFCD01310970

SMILES:

CC(C)[C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)NC1=CC2=C(C=C1)C(=CC(=O)O2)C(F)(F)F)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CC(=O)O)NC(=O)C

Synonym:

caspase-3 fluorogenic substrate ; n-acetyl-asp-glu-val-asp 7-amido-4-trifluoromethylcoumarin ; ac-asp-glu-val-asp-7-amino-4-trifluoro-methylcoumarin ; ac-asp-glu-val-asp-afc ; l-a-asparagine, n-acetyl-l-a-aspartyl-l-a-glutamyl-l-valyl-n-[2-oxo-4-(trifluoromethyl)-2h-1-benzopyran-7-yl]- ; caspase 3 (apopain) substrate 1f, fluorogenic ; fluorogenic caspase 3 substrate ac-devd-afc ; m.w. 729.62 c30h34f3n5o13 ; caspase-3 substrate vii, fluorogenic ; n-acetyl-asp-glu-val-asp-7-amino-4-trifluoromethylcoumarin afc ; ac-asp-glu-val-asp -7-amino-4-trrifluoromethylcoumarin ; ac-devd-afc ; caspase-3 substrate (afc) ; n-acetyl-asp-glu-val-asp-(7-amino-4-trifluoromethylcoumarin)

Description

Ac-DEVD-AFC is a fluorogenic substrate for caspase-3 (CPP32) and related cysteine proteases, which emits a blue to green shift in fluorescence. It also acts as a substrate for granzyme B-processed caspase-6, caspase-7, caspase-8, and caspase-10. It is known that Ac-DEVD-AFC has a greater Stokes' shift than Ac-DEVD-AMC once it has been cleaved. Since caspase-3 is an effector caspase, it is involved in the cleavage of many substrates which promote apoptosis. This indicator excites light at 400 nm and emits light at 505 nm. In addition, Ac-DEVD-AFC is modeled after Asp216 on caspase-3, the PARP cleavage site. Ac-DEVD-AFC is an essential indicator used to monitor the cellular effects of caspase-3.