Tris-Borate-EDTA buffer, 5X | CAS 610769-35-2

Element: borate buffer edta tris

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Identification

Tris-Borate-EDTA buffer, 5X

CAS:

610769-35-2

EINECS:

Molecular Formula:

C₁₄H₃₀BN₃O₁₄

MDL:

MFCD00236358

SMILES:

B(O)(O)O.C(CN(CC(=O)O)CC(=O)O)N(CC(=O)O)CC(=O)O.C(C(CO)(CO)N)O

Synonym:

tris borate edta buffer, 10x, dnase, rnase and protease free, ph 8.3, for molecular biology 1lt ; tris-borate-edta buffer, 5x ; tris borate edta buffer,for molecular biology,10x, dnase, rnase and protease free, ph 8.3 ; tris borate edta buffer, 10x, dnase, rnase and protease free, ph 8.3, for molecular ; tris borate edta 10x powder ; tris-borate-edta 10x solution ; tris borate edta buffer, 10x, dnase, rnase and protease free, ph 8.3, for molecular biology ; tbe buffer mix

Description

Tris-Borate-EDTA (TBE) buffer, 5X is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids. Since borate in TBE buffer is a strong inhibitor for many enzymes, TAE buffer (Tris Acetate-EDTA buffer, 10X) is recommended when looking at enzymatic applications for the DNA sample.

Suppliers

Santa Cruz Biotechnology, Inc.

296652

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CAS equal suppliers

TBE Buffer

Biosolve B.V.

BB2801120

Prices & Availability

Data is processed

Properties for Tris-Borate-EDTA buffer, 5X

Molecular Weight:

475.21 g·mol−1

Melting point:

170 °C

Boiling point:

219-220 °C/13HPA

Solubility:

soluble in water

Physical Description:

clear colourless solution

Hazard Statements

GHS03
Harmful

Acute toxicity (oral, dermal, inhalation), category 4
Skin irritation, categories 2, 3
Eye irritation, category 2A
Skin sensitization, category 1
Specific target organ toxicity following single exposure, category 3
- Respiratory tract irritation
- Narcotic effects

Not used

with the "skull and crossbones" pictogram
for skin or eye irritation if:
the "corrosion" pictogram also appears
the "health hazard" pictogram is used to indicate respiratory sensitization

GHS08
Health hazard

Respiratory sensitization, category 1
Germ cell mutagenicity, categories 1A, 1B, 2
Carcinogenicity, categories 1A, 1B, 2
Reproductive toxicity, categories 1A, 1B, 2
Specific target organ toxicity following single exposure, categories 1, 2
Specific target organ toxicity following repeated exposure, categories 1, 2
Aspiration hazard, categories 1, 2

Safety & Transport Information

Alternative Distributors of [Tris-Borate-EDTA buffer, 5X]

Producers or manufacturers change the product range from time to time. Currently there are no other manufacturers known.

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