L-1-Tosylamide-2-phenylethylchloromethyl Ketone

This product inhibits protease, and inactivates chymotrypsin but does not inactivate trypsin. It has been shown to irreversibly inhibit chymotrypsin-like serine proteases including ficin, bromelain, and papain. It has also been shown to inhibits I kappaB alpha-protease. Inhibition of I kappaB alpha-protease blocks the LPS- or cytokine-induced activation of nuclear factor kappaB (NF-kappaB), which then blocks iNOS induction and COX-2 transcription. It is reported to block apoptosis cell lines through the inhibition of the processing of caspases in some cell lines and to some stimuli. It can also be used in histone isolation.