3,3',5,5'-Tetramethylbenzidine Dihydrochloride Dihydrate | CAS 207738-08-7

Element: tetramethylbenzidine dihydrochloride dihydrate

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Identification

3,3',5,5'-Tetramethylbenzidine Dihydrochloride Dihydrate

CAS:

207738-08-7

EINECS:

Molecular Formula:

C₁₆H₂₀ N₂ . 2 Cl H . x H₂ O

MDL:

MFCD03065942

SMILES:

CC1=CC(=CC(=C1N)C)C2=CC(=C(C(=C2)C)N)C.O.O.Cl.Cl

Synonym:

3,3',5,5'-tetramethylbenzidine dihydrochloride hydrate 97% ; 3,3',5,5'-tetramethylbenzidine dihydrochloride hydrate vetec(tm) reagent grade, >=98% ; [1,1'-biphenyl]-4,4'-diamine,3,3',5,5'-tetramethyl-, dihydrochloride, hydrate (9ci) ; 3,3',5,5'-tetramethylbiphenyl-4,4'-diamine dihydrochloride hydrate (1:2:) ; tmb*2hcl*2h2o ; 3,3',5,5'-tetramethyl-[1,1'-biphenyl]-4,4'-diamine hydrochloride hydrate ; 3,3',5,5'-tetramethylbenzidine hydrate dihydrochloride ; tmb (hydrochloride hydrate) ; tmb 2hc1.xh2o ; [1,1'-biphenyl]-4,4'-diamine,3,3',5,5'-tetramethyl-, hydrochloride, hydrate (1:2:?) ; 3,3',5,5'-tetramethylbenzidine dihydrochloride hydrate, 98+% ; 3,3',5,5'-tetramethylbenzidine dihydrochloride hydrate,tmb dihydrochloride

Description

3,3',5,5'-Tetramethylbenzidine dihydrochloride dihydrate (TMB) is an aromatic amine that undergoes oxidation by the higher oxidation states of heme peroxidases (compounds I and II) thereby serving as a reducing co-substrate. One electron oxidation of TMB results in a radical cation that creates a charge-transfer complex with the unoxidized compound. This charge transfer complex absorbs at 652 nm (ε = 39,000). The completely oxidized form (diimine) absorbs at 450 nm (ε = 59,000) and is formed by two sequential one-electron oxidations of TMB. Thus the stoichiometry of oxidation is 0.5 mole charge transfer complex (λmax = 652 nm) or 1 mole of diimine (λmax = 450 nm) formed (or TMB oxidized) per mole of hydroperoxide reduced by the peroxidase.

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DC694427776

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