N-Succinimidyl-leucyl-leucyl-valyl-tyrosyl-Rh110-D-proline

This asymmetric Rhodamine 110 substrates for calpain employs a simple and straight forward kinetics upon analysis. Absorption wave length 492 nm, emission wave length 529 nm. While the intact substrate is virtually non-fluorescent, fluorescence largely increases (approx.. 3500 fold) upon cleavage. Superior signal-to-noise ratio combined with high sensitivity. In Rh110 assays this substrate delivers much less false positive hits in synthetic product libraries than corresponding AMC (7-amino-4-methylcoumarin) substrates. References: Chemical Synthesis of Ubiquitin, Ubiquitin-Based Probes, and Diubiquitin; Farid El Oualid, Remco Merkx, Reggy Ekkebus, Dharjath S. Hameed, Judith J. Smit, Annemieke de Jong, Henk Hilkmann, Titia K. Sixma, and Huib Ovaa; Angew. Chem. Int. Ed. 2010; 49: 10149 -10153. DOI: 10.1002/anie.201005995. C. Wiesmann et al.; J. Mol. Biol. 2012; 419: 4-21. S.K. Grant et al.; J. Biomol. Screen. 2002; 7(6): 531-540. U. Hassiepen et al.; Anal. Biochem. 2007; 371: 201-207. S.P. Leytus et al.; Biochem. J. 1983; 209: 299-307.