Pyronin Y

Pyronin Y is a dye that intercalates and stains RNA red, and has been shown to accumulate in the mitochondria of viable cells. Multidrug-resistant (MDR) Acinetobacter baumannii studies tested inhibition of the efflux pump by measuring the intracellluar accumulation of Pyronin Y and Ethidium bromide. Research shows that at low Pyronin Y concentrations the cytostatic effect is cell arrest in the G₁ phase, whereas at high concentrations the cells arrest is in the G₂ and S phase. Pyronin Y has been used in combination with Alcian Blue to asses and study pulmonary diseases, and in combination with Methyl Green (sc-215348) to stain nucleic acids in paraffin sections. Additionally Pyronin Y has been used to measure RNA in flow cytometry.