4-Nitrophenyl-N-acetyl-beta-D-glucosaminide, 99%

Chromogenic beta-Glucosaminidase substrate yielding a yellow solution upon cleavage, used particularly in yeasts and molds. 1. Conchie J., Hay A.J., Mammalian glycosidases. 4. The intracellular localization of beta-galactosidase, alpha-mannosidase, beta-N-acetylglucosaminidase and alpha-L-fucosidase in mammalian tissues. Biochem. J. 1963, 87, 354-61. 2. Drouillard S., Armand S., Davies G.J., Vorgias C.E., Henrissat B., Serratia marcescens chitobiase is a retaining glycosidase utilizing substrate acetamido group participation. Biochem. J. 1997, 328, 945-9. 3. Jones C.S., Kosman D.J., Purification, properties, kinetics and mechanism of beta-N-Acetylglucosaminidase from Aspergillus niger. J. Biol. Chem. 1980, 255, 11861-9. 4. Niimi K., Shepherd M.G., Cannon R.D., Distinguishing Candida species by beta-N-acetylhexosaminidase activity. J. Clin. Microbiol. 2001, 39, 2089-97. 5. Shibata H., Yagi T., Rate assay of N-acetyl-beta-D-hexosaminidase with 4-nitrophenyl N-acetyl-beta-D-glucosaminide as an artificial substrate. Clin. Chim. Acta 1996, 251, 53-64. 6. Steigerwald J.C., Bartholomew B.A., The assessment of lysosomal glycosidases in normal skin. Biochim. Biophys. Acta 1973, 321, 256-61.