4-Methylumbelliferyl-beta-D-glucuronic acid dihydrate, 99%

4-Methylumbelliferyl beta-D-glucuronide (MUG) is commonly used as a substrate for detecting beta-glucuronidase. MUG is used for identifying E. coli contamination in food, drinking water, clinical samples and for rapid bacterial identification in blood cultures. It is recommended in international normatives (ISO, DIN) as a fluorogenic substrate for detection of E. coli. MUG is also a tool assaying the glucuronidase gene in plants. 1. Feng P.C., Hartman P.A., Fluorogenic assays for immediate confirmation of Escherichia coli. Appl. Environ. Microbiol. 1982, 43, 1320-9. 2. Gould J., Nondestructive assay for beta-glucuronidase in culture media of plant tissue cultures. Methods Enzymol. 1992, , 357-62. 3. Jackson L., Langlois B.E., Dawson K.A., Beta-glucuronidase activities of fecal isolates from healthy swine. J. Clin. Microbiol. 1992, 30, 2113-7. 4. Jefferson R.A., Assaying chimeric genes in plants: The GUS gene fusion system. Plant Mol. Biol. Rep. 1987, 5, 387. 5. Kampfer P., Rauhoff O., Dott W., Glycosidase profiles of members of the family Enterobacteriaceae. J. Clin. Microbiol. 1991, 29, 2877-9. 6. Manafi M., New developments in chromogenic and fluorogenic culture media. Int. J. Food Microbiol. 2000, 60, 205-18. 7. Manafi M., Kneifel W., Bascomb S., Fluorogenic and chromogenic substrates used in bacterial diagnostics. Microbiol. Rev. 1991, 55, 335-48. 8. Mates A., Shaffer M., Membrane filtration differentiation of E. coli from coliforms in the examination of water. J. Appl. Bacteriol. 1989, 67, 343-6. 9. Sarhan H.R., Foster H.A., A rapid fluorogenic method for the detection of Escherichia coli by the production of beta-glucuronidase. J. Appl. Bacteriol. 1991, 70, 394-400. 10. Shadix L.C., Dunnigan M.E., Rice E.W., Detection of Escherichia coli by the nutrient agar plus 4-methylumbelliferyl beta-D-glucuronide (MUG) membrane filter method. Can. J. Microbiol. 1993, 39, 1066-70. 11. Trepeta R.W., Edberg S.C., Methylumbelliferyl-beta-D-glucuronide-based medium for rapid isolation and identification of Escherichia coli. J Clin Microbiol. 1984, 19, 172-4.