MAHMA NONOate

NOC-9 is a diazeniumdiolate (NONOate) small molecule NO donor with a short NO release half-life of 1 min at 37°C and 3 min between 22 and 25°C. NOC-9 will deliver a quick burst of NO to the system, and is useful for investigation of the effect on cells and tissues of acute NO exposure. Both NOC-9 and NOC-18 are believed to prevent low density lipoprotein peroxidation by Cu²⁺, although the quicker release of NOC-9 displayed less effective protection against LDL peroxidation than NOC-18 (half-life 20 hr). NOC-9 has also been used to elucidate the bifunctional effect of NO on skeletal muscle Ca²⁺ release channels. Oxidation of thiol moeities to form disulfide bonds across subunits of the Ca²⁺ release channels leads to an increase in channel activity. Low concentrations of NO resulting from slow release from the donor or low concentration of the donor blocks this oxidation and attenuates channel activity. High concentrations of NO, provided in the report by the rapid release of NO from NOC-9, demonstrates oxidation of the thiols and an increase in Ca²⁺ channel activity.