3-Indoxyl-beta-D-glucoronic acid cyclohexylamine salt, 98%

Chromogenic substrate for beta-D-glucuronidase yielding a blue precipitate upon cleavage. It is used in detection and enumeration of E. coli. 1. Brenner K.P., Rankin C.C., Roybal Y.R., Stelma G.N. Jr., Scarpino P.V., Dufour A.P., New medium for the simultaneous detection of total coliforms and Escherichia coli in water. Appl. Environ. Microbiol. 1993, 59, 3534-3544. 2. Brenner K.P., Rankin C.C., Sivaganesan M., Scarpino P.V., Comparison of the recoveries of Escherichia coli and total coliforms from drinking water by the MI agar method and the U.S. Environmental Protection Agency-approved membrane filter method. Appl. Environ. Microbiol. 1996, 62, 203-8. 3. Haines J.R., Covert T.C., Rankin C.C., Evaluation of indoxyl-beta-D-glucuronide as a chromogen in media specific for Escherichia coli. Appl. Environ. Microbiol. 1993, 59, 2758-9. 4. Ley A.N., Bowers R.J., Wolfe S., Indoxyl-beta-D-glucuronide, a novel chromogenic reagent for the specific detection and enumeration of Escherichia coli in environmental samples. Can. J. Microbiol. 1988, 34, 690-3.