Indole-2-carboxylic acid, 99%

Substrate for decarboxylase. The formation of indole can be detected by monitoring absorbance. Was used as chromogenic substrate for cloning genes encoding dioxygenases that act on aromatic acids. The p-cumate-degrading strain Pseudomonas putida F1 and the m- and p -toluate-degrading strain P. putida mt-2 transform indole-2-carboxylate and indole-3-carboxylate to colored products identified as indigo, indirubin, and isatin. Dioxygenase is thought to catalyse the first step in this reaction. It is also a competitive antagonist of potentiation by glycine at the NMDA receptor. 1. Eaton R.W., Chapman P.J., Formation of indigo and related compounds from indolecarboxylic acids by aromatic acid-degrading bacteria: chromogenic reactions for cloning genes encoding dioxygenases that act on aromatic acids. J. Bacteriol. 1995, 177, 6983-8. 2. Huettner J.E., Indole-2-carboxylic acid: a competitive antagonist of potentiation by glycine at the NMDA receptor. Science 1989, 243, 1611-3. 3. Smith D.H., Okiyama K., Thomas M.J., McIntosh T.K., Effects of the excitatory amino acid receptor antagonists kynurenate and indole-2-carboxylic acid on behavioral and neurochemical outcome following experimental brain injury. J. Neurosci. 1993, 13, 5383-92.