DIPSO

DIPSO is a Zwitterionic buffer useful in the pH range of 7.0-8.2. Studies show that using one buffer limits the ability to adjust the range of buffering capacity. To increase the range of buffering capacity research suggests that it may be beneficial to combine multiple buffers such as HEPES, MOPS and DIPSO. This buffer combination will allow the researcher the ability to expand pH buffering over a range of temperatures while decreasing the individual concentrations of buffers used, thus lowering the chance of toxicity.