(S)-4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid, Firefly, potassium salt

Water soluble substrate for firefly luciferase. Used for imaging the expression of the luc marker gene and luciferase-fusion conjugates in living cells, tissues and organisms. Extensivly utilized in reporter gene assays, immunoassays and hygiene monitoring. 1. Campbell A.K., et al., Bioluminescence and Chemiluminescence: Fundamental and Applied Aspects. John Wiley, Chichester, 1994. 2. Chappelle E.W., et al, Determination of bacterial content in fluids. Methods Enzymol. 1978, , 65. 3. DeLuca M. (Ed.), Purification and properties of firefly luciferase. Methods Enzymol. 1978, 57, 5. 4. Gould S.J., Subramani S., Clinical and biochemical applications of luciferases and luciferins. Anal. Biochem. 1988, 175, 5-13. 5. Greer L.F. III, Szalay A.A., Imaging of light emission from the expression of luciferases in living cells and organisms: a review Luminescence 2002, 17, 43-74. 6. Karl D.M., Determination of GTP, GDP and GMP in cell tissue extracts. Methods Enzymol. 1978, 57, 85. 7. Kricka L.J., Clinical and biochemical applications of luciferases and luciferins. Anal. Biochem. 1988, 175, 14-21. 8. Kricka L.J., Literature Review Luminescence 2002, 17, 168-78.