(Carboxymethyloximino)spiperone, Hemihydrate

(Carboxymethyloximino)spiperone, Hemihydrate is used as an affinity chromatography matrix support for the purification of the D₂-dopamine receptor. Spiperone is an 5-HT_2A serotonin and selective D₂-like dopamine receptor antagonist (K_i values are 0.06, 0.6, 0.08, ~ 350, ~ 3500 nM for D₂, D₃, D₄, D₁ and D₅ receptors respectively). Although the well known role of Spiperone in research studies indicates it acts as an antagonist of either 5-HT₂ or D₂ receptors, Spiperone has also been reported to act through a protein tyrosine kinase-coupled phospholipase C-dependent pathway. Spiperone was observed to act independent of either 5-HT₂ or D₂ receptors as reported In a study investigating the role of Spiperone and Ca²⁺ activated Cl^- (CaCC) in human cystic fibrosis (CF) airway epithelial cell monolayers in vitro.