BAPTA-APM

BAPTA-APM [5-(3-Aminopropyl)-5'-methyl-bis-(2-aminophenoxy-methylene)-N,N,N',N'-tetraacetate, tetrapotassium salt] is a fluorescent chelating indicator used to study cytosolic free calcium. Fluorescent calcium indicators selectively bind to calcium ions and show a spectral response upon binding Ca²⁺; thus, fluorescence intensity or wavelength are altered. This shift of fluorescence wavelength and change of intensity upon calcium chelation can be monitored using fluorescence microscopy. This high quality fluorescent Ca²⁺ chelator is useful for many intracellular applications, especially in single cells, adherent cell layers, or bulk tissues.