L-Arginine 7-amido-4-methylcoumarin dihydrochloride

Excellent sensitive fluorogenic substrate for the quantitative determination of cathepsin H activity. Also useful as a substrate for aminopeptidase B, aminopeptidase in common, pepN, pepC, Trypsin. Yields a blue fluorescent solution upon cleavage. 1. Barrett A.J., Fluorimetric assays for cathepsin B and cathepsin H with methylcoumarylamide substrates. Biochem. J. 1980, 187, 909-12. 2. Barrett A.J., Kirschke H., Cathepsin B, Cathepsin H, and Cathepsin L. Methods Enzymol. 1981, 80, 535-61. 3. Sanz Y., Toldrá F., Purification and Characterization of an Arginine Aminopeptidase from Lactobacillus sakei. Appl. Env. Microbiol. 2002, 68, 1980-7. 4. Schwartz W.N., Barrett A.J., Human Cathepsin H. Biochem. J. 1980, 191, 487-97.